How Strong is Your Binding? A Quick Introduction to Isothermal Titration Calorimetry
What is Isothermal Titration Calorimetry? Isothermal titration calorimetry (ITC) measures the heat generated (or absorbed) when one solution is titrated into another. Most commonly, a small molecule or peptide is titrated into a protein. If the molecule binds to the protein, heat is given off (or absorbed) with each injection, until the protein is saturated.1…
