Methods for Relative Quantification of qPCR Data: Yes, There is More Than One
Relative Quantification in qPCR compares gene expression levels using either the ΔΔCt or Pfaffl methods. Both rely on housekeeping genes for normalization but differ in handling primer efficiency. The ΔΔCt method assumes equal efficiency, while the Pfaffl method corrects for differences. Selecting stable reference genes or using multiple genes improves accuracy. Understanding these methods helps researchers accurately analyze gene expression changes in their experiments.
