DNA plasmid preps

Image of a colorful spring toy depicting coils in DNA plasmid preps to help undertand why you get 3 bands when running plasmid DNA on agarose gels

DNA / RNA Manipulation and Analysis

How to Identify Supercoils, Nicks and Circles in DNA Plasmid Preps using Gel Electrophoresis

ByDr Rebecca Tirabassi

Why do you get three bands when running uncut plasmid DNA on agarose gels. Discover the answer and how it can help improve your DNA plasmid preps.

An image of different sized leaves to depict kit-free midiprep.

DNA / RNA Manipulation and Analysis

How to Do a Kit-free Midiprep

ByVicki Doronina

Here, we share a protocol for a midiprep, which, if not faster, gives a larger plasmid DNA yield than any commercial midiprep kit.

An image of neon signs to depict a menagerie of miniprep methods.

DNA / RNA Manipulation and Analysis

A Menagerie of Miniprep Methods

ByLiam Thompson

There’s more than one way to do a plasmid miniprep. Here are 5 to add to your molecular biology arsenal.

10 Things Every Molecular Biologist Should Know

The eBook with top tips from our Researcher community.