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BLAST

A Crash Course in BLAST Searching
Genomics and Epigenetics

A Crash Course in BLAST Searching

ByVivek Thiruvettai

Simple BLAST searching is pretty straightforward to many of us. Just plug in your sequence, select the species genome, and hit search! But have you ever wondered what it takes to run a BLAST query using these mammoth-sized (no pun intended!) sequence databases? BLAST searching can produce dozens, hundreds, or even thousands of candidate alignments….

Read More A Crash Course in BLAST SearchingContinue

Have a BLAST With Your Protein Sequences
Protein Expression and Analysis

Have a BLAST With Your Protein Sequences

ByBrian McWilliams

When I was being trained in microbiology as an undergrad, one of the first skills I acquired was the ability to quickly compare and visualize amino acid sequences using BLAST and ClustalW. 15 years later, those two programs have done nothing but improve by expanding the data contained in these databases and simplifying the user…

Read More Have a BLAST With Your Protein SequencesContinue

Time to Instigate Nested PCR
PCR, qPCR and qRT-PCR

Time to Instigate Nested PCR

ByOlwen Reina

How to Obtain a Purer PCR Product and Reduce Non-specific Amplification Unless you’ve gotten your hands on some miraculously specific primers, amplification of only your target sequence without non-specific amplification can be very challenging. Thankfully, a clever and surprisingly simple solution is at hand! A Quick Recap of the Basics In PCR, you design your…

Read More Time to Instigate Nested PCRContinue

Software and Online Tools

BLAST OFF! The Basic Local Alignment Search Tool Explained

ByDimitris Skliros

BLAST is the most popular bioinformatics online tool. But in order to properly use it, you have to understand that is not just a search query against a database but something far more complicated, and the information you get back far more…precious!

Read More BLAST OFF! The Basic Local Alignment Search Tool ExplainedContinue

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