QED Bioscience

Taking up the Challenges of In Vitro Monoclonal Antibody Production

Monoclonal antibodies are extensively used in research laboratories, diagnostic products and immunotherapy and have multiple advantages over polyclonal antibodies. They exhibit enhanced specificity to single epitopes, have little or no variability, and are easy to modify and customize as required. The History of Monoclonal Antibodies In 1984, Georges Köhler, César Milstein, and Niels Jerne received…

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How to Obtain Stellar Staining with Fluorescent IHC

They say “a picture is worth a thousand words.” This is especially true in the case of fluorescent immunohistochemistry (IHC). With the flip of a microscope filter, you can gaze into a brightly colored galaxy of red, green, and blue pixels, waiting to be captured and presented in your next publication. If you are interested in…

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ELISA: How I Wonder What You Are

My phone’s email notification went off, and I rolled over in bed to look at the clock. Saturday, 5 am. Wonderful. Who would email me at that hour? It had to be my undergraduate research PI. I unlocked my phone. Yep. Doesn’t he ever sleep? Dear Casey: We are launching a new collaborative project in…

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How to Choose Quality Antibodies for Successful Western Blotting

Successful western blotting means achieving unambiguous results, and this requires a sensitive and specific antibody-antigen interaction. Consequently, high quality antibodies are critical for reliable and consistent western blotting. Western Blotting Process In the basic western blotting process, polyacrylamide gel electrophoresis (PAGE) separates a mix of proteins according to their molecular weights (denaturing gels) or their…

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