Expansion Microscopy: Achieving Nanoscale Resolution Using Conventional Fluorescence Microscopes
For decades, the diffraction of light has placed a hard limit on optical microscopy. Conventional widefield and confocal microscopes can’t resolve features smaller than about 200 nm laterally, which means critical signaling compartments and the nanoscopic organization of synapse-associated molecules remain hidden. The 21st century brought revolutionary super-resolution technologies, such as stimulated emission depletion (STED),…
