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So far in this series I have given you an overview of how STED works and how to design your STED experiments. In this final article, I will tell you how to do two color STED and go over some tips and tricks to acquire the best images from your sample. Dual Color STED Fluorophore…
In part 1, “The Power of STED Microscopy: How Does it Work?” I covered the basics of STED Microscopy: how it works and why you might want to use it. If you now want to do STED Microscopy, the next step is to optimize your sample. In this article, I cover how you can get…
Do you suspect that your favourite protein is doing something really cool? But you cannot see it because your confocal microscope’s resolution is limited. Then Stimulated Emission Depletion (STED) microscopy is what you need! With the power to smash through the diffraction limit of confocal microscopy, STED opens up a whole new world of improved…
Single-molecule localization techniques are another way to achieve super resolution. Discover how the different variants work, and get info on what researchers are doing with them.
Super resolution microscopy shattered the lower resolution limit offered by traditional confocal microscopy, enabling us to see more detail. Discover the different super resolution techniques, how they work, and what they are being used for.
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