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There are several methods you can use to see if your T cells are cytotoxic, but a chromium release assay using radioactive 51chromium (51Cr) is one of the oldest. It gives good results, and is great for labs that can’t afford or don’t have flow cytometry readily available. Here, I will outline a simple method…
Let’s face it, when you leave the lab for the day, your mind is still racing with ideas and questions about your experiment: how to fix this or what went wrong or what does this data point even mean. It can be difficult to actually shut down and decompress when you walk out of the…
In the midst of all the cool new sequencing techniques and technologies out there today, you may have overlooked the tried and true method of Shotgun Sequencing. What is Shotgun Sequencing Anyway? Shotgun sequencing gets its name from the concept that a large sequence is essentially broken up in to many, many smaller pieces, similar…
If you’ve ever had backache from sitting at the microscope or biosafety cabinet, these tips may be useful for you!
Let’s face it, at least once in your lab life you are going to need a favor. You may need to go on vacation, you may be sick or you may just be a little overwhelmed at the bench. At least once, you will need to ask for a little help and someone will surely…
Sometimes, you have to work with radioactive materials in the lab. If you happen to be one of those lucky individuals, it is important to take extra precautions in your work to ensure your safety, and good results! Here are my tips to make sure you radiate confidence doing your experiments. Know Your Element It…
While most of us have heard of super resolution microscopy, many of you may not have heard of MSIM, or Multifocal Structured Illumination Microscopy. This under-the-radar imaging technique is relatively quick, cheap (by comparison) and will allow you to get a lot of data, fast. So What is MSIM Anyway? MSIM, as I mentioned earlier,…
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