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last updated: February 17, 2025
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Simple Tips for Model Organism-Based Work The mouse is the favored model organism for life science researchers so much so that mice account for about 95% of all lab animals used in research. The striking similarities between the human and mouse genomes, ease of genetic manipulation and the uniformity achieved through inbred mating makes them…
As a protein biochemist where bacteria were mere workhorses, imagine my surprise when I began work in a bonafide micro lab! I discovered that bacteria could be much fussier than my good ol’ cloning and expression friends E. coli DH5ɑ or BL21. One broth would not do for all, some even required blood! No, no,…
Does your laboratory resemble the nest of an overly enthusiastic laboratory rat that went on a scavenger hunt and squirrelled away all that it has found? Do you find yourself playing Jenga with stacks of Petri dishes and freezer boxes? Have you ever attempted to decipher the meaning of the mysterious string of numbers on…
Mycoplasma infections are very, very bad news; these special prokaryotes can rapidly spread through your cell culture and inhibit cell proliferation, induce apoptosis, cytokines and radicals, and otherwise transform your cells. Worst of all, since contamination is not easy to spot, you may not realize your culture is contaminated until it’s too late. The 100…
It’s official. You’ve signed on the dotted line and you are about to begin the most exciting and frustrating journey into the depths of the unknown: a journey otherwise known as a PhD. You’ve heard the horror stories from previous students; the cloning that wouldn’t work for reasons unknown to man, the data that indicates…
Fortunately for microscopy users, measuring intracellular fluorescence has been made relatively simple through an ImageJ plugin called the Cell Magic Wand. For those of you unfamiliar with ImageJ, it’s a popular image processing program that runs on Mac, Windows, and Linux. How to use ImageJ for measuring intracellular fluorescence First of all, to begin measuring…
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