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The gap year I intended to take between my Master’s degree and hypothetical Ph.D. is now going into its 4th year. Here’s why I’m not worried. These days it seems like undergraduates are proceeding en masse to graduate programs shortly after completing their senior year of college. An abundance of undergraduate research opportunities and poor…
Pulling an over-nighter is a common theme for many of my college friends. I, however, like my sleep—particularly between midnight and 7 am. Therefore, I tend not to stay up late to study and avoided jobs that required me to work a night shift. Then, I went to grad school and had to put in…
P-value abuse directly contributes to one of the biggest problems facing the scientific community: the prominence of false-positive results in the published literature. Contrary to popular interpretation, the p-value doesn’t indicate the likelihood that the observed result was due to chance. There are important qualifications to p-value interpretation. Moreover, the p-value cannot directly speak to…
Journal Club. So much more than reading a paper aloud. So many ways to mess it up. Got to present one? Then read our journal club toolkit.
When I began a master’s program in 2008, the lab task I hated more than anything was running agarose gels for DNA. Something so simple, ubiquitous, and necessary gobbled up more hours in the lab than I care to remember. Even though we added the DNA stain directly to the molten agarose and didn’t have…
In the previous installment of this series on western blotting, we addressed potential sources of error when your final product is completely bare. But alternatively, what do you do when too much background is the problem? You may have beautiful bands of interest—but if there is a bunch of non-specific binding, your quantification and data…
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