Techniques / Flow Cytometry

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Are Quantum Dots Any Good for Flow Cytometry?

What Are Quantum Dots? Quantum dots were discovered in the early 1980s. However, it was not until the late 1990s that their use in biological applications was suggested.1 Quantum dots are semiconducting nanocrystals made of artificial atom clusters. Their size generally ranges from 2 to 20 nm. Size is crucial for their physical properties because […]

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In Flow Cytometry 23rd of March, 2017
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Hierarchical or Boolean Gating: Which One to Choose?

A flow cytometer collects the events you are interested in, and also ‘sees’ every event that goes through. This includes debris and even bits in your buffers. As cytometrists, we gate our cells to exclude unwanted bits and to focus on the sub-populations that we are interested in studying. There are two main ways of gating […]

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In Flow Cytometry 14th of February, 2017
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BD OptiBuild™ Custom Reagents are Unlocking Science with New Antibody and Dye Combinations

The need for multicolor flow cytometry is increasing all the time. We want to look at more markers, in smaller samples, perform quicker acquisition, have simpler post-acquisition analysis and, oh wait, can we please just add in yet another color? Sometimes it’s not that easy, since not all the reagents in your panel are available […]

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In Flow Cytometry 22nd of July, 2016