TEER

Before running MDCK transport assays, don’t rely on visual confluency alone. A monolayer may look ready but still be leaky, leading to misleading permeability data. Validate MDCK monolayer integrity first, using TEER to assess electrical resistance and lucifer yellow passage to detect leakage between cells. Together, these checks help confirm whether each well is suitable for transport measurements before you invest time and valuable compounds in the assay.