SAGE Part 2: LongSAGE, RL-SAGE and SuperSAGE

SAGE Part 2: LongSAGE, RL-SAGE and SuperSAGE

ByOlwen Reina

SAGE, or serial analysis of gene expression, is a technique that enables you to digitally analyze the entire gene expression profile of a cell(s). Before this technique, scientists were limited to studying a few gene’s expression at once by a technique called the expressed sequence tag approach. The coolest part of SAGE is you don’t…

Vital for Soup, Vital for Labs: Serial Analysis of Gene Expression (SAGE), part 1

Vital for Soup, Vital for Labs: Serial Analysis of Gene Expression (SAGE), part 1

ByOlwen Reina

Some techniques can sound very dry but this isn’t one of them! SAGE was first described and published by Velculescu et al. in 1995. At the time, techniques like RNA blotting and expressed sequence tagging were used to study gene expression. However techniques like these were slow and very limited. The speed of SAGE and…