Interpreting Cellular Metabolism Assays: How Metabolic Crossroads Reveal What Cells Are Really Doing
When interpreting cellular metabolism assays, focusing on single pathways can be misleading, as metabolism operates as a connected network. Metabolites such as pyruvate, malate, glycogen, branched-chain amino acids, and β-hydroxybutyrate sit at key metabolic crossroads, where changes reflect shifts in energy routing, redox balance, and stress adaptation. Understanding these junctions helps you evaluate metabolic data more accurately and design experiments that capture the true cellular state.
