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Achieving publication-quality immunofluorescence images is tricky. Learn what controls for immunofluorescence you can use to get them!
Autofluorescence, background fluorescence in unstained cells or tissues, often interferes with microscopy clarity. The article outlines causes, such as natural fluorophores like NADH, and offers strategies like selecting distinct fluorophores and optimizing sample prep to minimize its impact and enhance image accuracy.
If you have ever imaged biological samples, you have likely encountered autofluorescence. That pesky background coloration you see under the microscope, which can make it difficult to distinguish your actual signal from the noise.1 When you are trying to look for something as delicate as RNA, you don’t want to be hunting for your signal…
The human brain autofluoresces—a funny thought next time you see a cartoon character with a bright idea and a light bulb over his head—but not so funny if you are attempting immunofluorescence analysis. But there are some significant advantages to using fluorescence detection over chromogenic methods. In this article, I will cover the advantages of…
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