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Immunohistochemistry isn’t just a useful clinical tool, it also has great applications as a basic research tool. We’ll walk you through the immunohistochemistry basics to get you off to a flying start.
In any application that uses antibodies for signal detection (e.g., Western blotting, ELISA, immunohistochemistry, or FACS), there are two approaches to antibody labeling: direct and indirect labeling. Standard Western blotting uses indirect labeling because you use a primary antibody to detect the target antigen, followed by a secondary antibody to which a detection molecule is…
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