Leica
Visualising protein degradation and aggregation in the living cell
Register Now In this webinar, Prof. Eric Reits will discuss how several neurodegenerative disorders, including Alzheimer’s, Parkinson’s and Huntington’s disease, are characterized by the formation of protein aggregates, and that components of the ubiquitin-proteasome system (UPS) co-localize in these aggregates. This has led to speculation that the UPS is impaired in these disorders, with UPS…
Read More Life beyond the pixels: deep learning methods for single cell analysis
In this webinar, you will discover: the computational steps in the analysis of single cell-based large-scale microscopy experiments; a novel microscopic image correction method designed to eliminate illumination and uneven background effects; new single-cell image segmentation methods; the Advanced Cell Classifier, a machine learning software tool for identifying cellular phenotypes; how the above machine learning…
Read More See The Hidden: Virtual Neurosciences Workshop
See The Hidden: Virtual Neurosciences Workshop This live online event brings together a series of scientific talks and microscopy showcases, centering around four specific topics in microscopy – 3D real time imaging*, laser microdissection, confocal nanoscopy and high pressure freezing techniques in electron microscopy. Each section will kick off with a scientific talk from one…
Read More Exploring The Structure and Life Cycle of Viruses
Exploring The Structure and Life Cycle of Viruses The Virologist’s Toolbox for Electron Microscopy Specimen Preparation Available On Demand Speakers Frédéric Leroux Advanced Workflow SpecialistLeica Microsystems Frédéric Leroux completed his Master degree in Biology in 2007 at the University of Ghent where he gained experience in biological EM sample preparation. In 2008, he moved to…
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Revealing functional mechanisms of synaptic transmission by high-pressure freezing
Read MoreFrom Organs to Tissues to Cells: Analyzing 3D Specimens with Widefield Microscopy
From Organs to Tissues to Cells: Analyzing 3D Specimens with Widefield Microscopy On Demand Speakers Dr. Falco Krüger Advanced Workflow Specialist Widefield Life Science Research DivisionLeica Microsystems Falco did his PhD in Biology at the Plant Cell Biology chair of Prof. Karin Schumacher at the Centre for Organismal Studies (COS) in Heidelberg, Germany. For his…
Read More Revealing functional mechanisms of synaptic transmission by high-pressure freezing
Revealing functional mechanisms of synaptic transmission by high-pressure freezing On Demand Speakers Frédéric Leroux Advanced workflow specialist, Leica Microsystems Frédéric Leroux completed his Master degree in Biology in 2007 at the University of Ghent where he gained experience in biological EM sample preparation. In 2008, he moved to the physics department at the University of…
Read More Workflows and instrumentation for cryo-electron microscopy: an overview
Workflows and instrumentation for cryo-electron microscopy: an overview Now On Demand Speaker Frédéric Leroux Advanced workflow specialist, Leica Microsystems Frédéric Leroux completed his Master degree in Biology in 2007 at the University of Ghent where he gained experience in biological EM sample preparation. In 2008, he moved to the physics department at the University of…
Read More THUNDER Imagers: High performance, versatility and ease-of-use for your everyday imaging workflows.
THUNDER Imagers: High performance, versatility and ease-of-use for your everyday imaging workflows. In this webinar, you will learn: How to get sharp, high quality images from thick 3-dimensional microscope samples How to shorten your imaging workflow from image acquisition to data analysis A broad spectrum of imaging workflows for a range of life science applications…
Read More Basics of Fluorescence Lifetime Imaging and FLIM Applications to FRET Biosensors
Basics of Fluorescence Lifetime Imaging and FLIM Applications to FRET Biosensors In this tutorial you will learn: How researchers (Anzilotti 2019) used FLIM to elucidate the mechanisms responsible for a B cell deficiency A new approach to measure the cellular function of the Zn2+ transporter ZIP7 in primary mouse cells, comparing wild-type and CRISPR-Cas9 derived mutant cells…
Read More Array Tomography for SEM 3D Reconstruction
Array Tomography for SEM 3D Reconstruction Speakers Frank Assen Robert Ranner Tutorial abstract Array tomography (AT) is a 3D image reconstruction technique for high resolution, quantitative analysis of biological structures. For optimal results, ultrathin and ordered sections are an absolute requirement. In this webinar you will get tips and tricks to optimize the workflow of…
Read More Expanding the Limits of Electron Microscopy Sample Preparation with the Leica EM ICE High Pressure Freezer
Expanding the Limits of Electron Microscopy Sample Preparation with the Leica EM ICE High Pressure Freezer In this tutorial you will learn: Improved vitrification: Specimen vitrification without synchronisation fluid Vitrification strategies: Optimized freezing for different techniques Light and electrical stimulation: Dissect cellular processes with millisecond precision A look into the future: freezing of crystals Speakers…
Read More Live Cell Isolation by Laser Microdissection
Live Cell Isolation by Laser Microdissection In this tutorial, you will learn: How to best prepare your specimen for live cell isolation by laser microdissection How to optimize your laser microdissection workflow How to avoid common pitfalls of this technique Speakers Dr. Oleg Podgorny Tutorial abstract Laser microdissection is a tool for the isolation of…
Read More LAS X Navigator Tutorial: Navigating live cell imaging
LAS X Navigator Tutorial: Navigating live cell imaging Speakers Dr. Avi Jacob Tutorial abstract Join Dr. Avi Jacob as he introduces you to the Leica LAS X Navigator, a software module that makes multiparametric acquisition and data review of large data sets easier than you ever thought possible! In this tutorial, Dr. Jacobs will discuss:…
Read More Super-resolved STED spectroscopy: New insights into molecular membrane dynamics
Super-resolved STED spectroscopy: New insights into molecular membrane dynamics Speakers Christian Eggeling Tutorial abstract Molecular interactions are key in cellular signalling. They are often ruled or rendered by the mobility of the involved molecules. We present different tools that are able to determine such mobility and potentially extract interaction dynamics. Specifically, the direct and non-invasive…
Read More Precise and lightning-fast spectral Fluorescence Lifetime Imaging at video rate integrated in a high-end confocal microscope
Precise and lightning-fast spectral Fluorescence Lifetime Imaging at video rate integrated in a high-end confocal microscope Speakers Kees JalinkProfessor, The Netherlands Cancer Institute Tutorial abstract Förster (Fluorescence) Resonance Energy Transfer (FRET) has become a powerful tool to study protein-protein interactions and signal transduction in living cells. FRET is commonly read out either by detecting the…
Read More How to Conduct Localized Proteomics of Microscopic Regions
Precise and lightning-fast spectral Fluorescence Lifetime Imaging at video rate integrated in a high-end confocal microscope In this tutorial, you will learn: How to perform isolated proteomics of microscopic regions of interest How to use this technique with FFPE tissue How proteomics can help you understand molecular mechanisms in disease Speakers Dr. Eleanor Drummond Tutorial abstract…
Read More Enhance Your Imaging with Super-Resolution: Improve Spatial Resolution and Increase Signal to Noise Ratio in Difficult Samples
In these informative case studies, you will learn how: HyVolution 2 can improve the spatial resolution you get from your sample, whilst remaining gentle to the fluorophores HyVolution 2 overcomes issues experienced with other imaging modalities, even in difficult samples such as plant material, increasing resolution and signal to noise ratio, thereby facilitating downstream image…
Read More Revealing Cellular Dynamics with Millisecond Precision – The New Tool That Turned Electron Micrographs into Motion Pictures of Neural Communication
Revealing Cellular Dynamics with Millisecond Precision – The New Tool That Turned Electron Micrographs into Motion Pictures of Neural Communication In this tutorial: What if you can dissect the cellular dynamics with millisecond precision? What if you can unravel the morphological transformation of a neuron millisecond by millisecond using electron microscopy? Could this be even…
Read More Using FIT probes and Super-Resolution Microscopy to Decipher Steps of mRNP Assembly in Developing Oocytes
In this webinar, presented by Dr. Imre Gaspar of the Developmental Biology Unit at EMBL, Heidelberg, you will learn: Importance of mRNA localization and function of mRNPs. Advantages of using fluorogenic FIT probes to visualize mRNPs in vivo and in fixed specimen. How super-resolution microscopy can identify factors required for mRNP biogenesis. Abstract: All mRNA…
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