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High-resolution 3D imaging uncovers vascular control of tissue ageing

High-resolution 3D imaging uncovers vascular control of tissue ageing Wednesday 15th September 2021LONDON 16:00 BST | BERLIN 17:00 CET | DUBAI 19:00 GST Ageing is associated with alterations in tissue homeostasis and a decline in tissue functions. Blood vessels provide supportive microenvironments for various cell types to maintain tissue homeostasis and organ function. However, ageing…

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See The Hidden: Artificial Intelligence in Microscopy

See The HiddenArtificial Intelligence in Microscopy Available On-Demand Introduction to AI for Microscopists: all you need to know Machine and deep learning technologies already play a major role in everyday life – think Alexa, Tesla’s Autopilot, Spotify, Amazon or YouTube. In the last 3 years, microscopists have started to use “AI based” solutions for image…

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Intravital Microscopy of Cancer

Intravital Microscopy of Cancer Available on Demand In this webinar, you will learn: about intravital microscopy techniques that allow you to monitor stem cells and cancer cells in real-time in vivo; how these techniques can help you study the identity and migratory behavior of cancer stem cells; how intravital microscopy uses multiphoton confocal microscopy to…

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Visualising protein degradation and aggregation in the living cell

Visualising protein degradation and aggregation in the living cell Available On Demandat MicroscopyFocus.com Watch Now In this webinar, Prof. Eric Reits will discuss how several neurodegenerative disorders, including Alzheimer’s, Parkinson’s and Huntington’s disease, are characterized by the formation of protein aggregates, and that components of the ubiquitin-proteasome system (UPS) co-localize in these aggregates. This has…

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Life beyond the pixels: deep learning methods for single cell analysis

Life beyond the pixels:Deep learning methods for single cell analysis Available On Demand In this webinar, you will discover: the computational steps in the analysis of single cell-based large-scale microscopy experiments; a novel microscopic image correction method designed to eliminate illumination and uneven background effects; new single-cell image segmentation methods; the Advanced Cell Classifier, a…

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See The Hidden: Virtual Neurosciences Workshop

See The Hidden: Virtual Neurosciences Workshop This live online event brings together a series of scientific talks and microscopy showcases, centering around four specific topics in microscopy – 3D real time imaging*, laser microdissection, confocal nanoscopy and high pressure freezing techniques in electron microscopy. Each section will kick off with a scientific talk from one…

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Exploring The Structure and Life Cycle of Viruses

Exploring The Structure and Life Cycle of Viruses The Virologist’s Toolbox for Electron Microscopy Specimen Preparation Available On Demand Speakers Frédéric Leroux Advanced Workflow SpecialistLeica Microsystems Frédéric Leroux completed his Master degree in Biology in 2007 at the University of Ghent where he gained experience in biological EM sample preparation. In 2008, he moved to…

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on demand

Revealing functional mechanisms of synaptic transmission by high-pressure freezing

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From Organs to Tissues to Cells: Analyzing 3D Specimens with Widefield Microscopy

From Organs to Tissues to Cells: Analyzing 3D Specimens with Widefield Microscopy On Demand Speakers Dr. Falco Krüger Advanced Workflow Specialist Widefield Life Science Research DivisionLeica Microsystems Falco did his PhD in Biology at the Plant Cell Biology chair of Prof. Karin Schumacher at the Centre for Organismal Studies (COS) in Heidelberg, Germany. For his…

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Revealing functional mechanisms of synaptic transmission by high-pressure freezing

Revealing functional mechanisms of synaptic transmission by high-pressure freezing On Demand Speakers Frédéric Leroux Advanced workflow specialist, Leica Microsystems Frédéric Leroux completed his Master degree in Biology in 2007 at the University of Ghent where he gained experience in biological EM sample preparation. In 2008, he moved to the physics department at the University of…

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Workflows and instrumentation for cryo-electron microscopy: an overview

Workflows and instrumentation for cryo-electron microscopy: an overview Now On Demand Speaker Frédéric Leroux Advanced workflow specialist, Leica Microsystems Frédéric Leroux completed his Master degree in Biology in 2007 at the University of Ghent where he gained experience in biological EM sample preparation. In 2008, he moved to the physics department at the University of…

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THUNDER Imagers: High performance, versatility and ease-of-use for your everyday imaging workflows.

THUNDER Imagers: High performance, versatility and ease-of-use for your everyday imaging workflows. In this webinar, you will learn: How to get sharp, high quality images from thick 3-dimensional microscope samples How to shorten your imaging workflow from image acquisition to data analysis A broad spectrum of imaging workflows for a range of life science applications…

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Basics of Fluorescence Lifetime Imaging and FLIM Applications to FRET Biosensors

Basics of Fluorescence Lifetime Imaging and FLIM Applications to FRET Biosensors In this tutorial you will learn: How researchers (Anzilotti 2019) used FLIM to elucidate the mechanisms responsible for a B cell deficiency A new approach to measure the cellular function of the Zn2+ transporter ZIP7 in primary mouse cells, comparing wild-type and CRISPR-Cas9 derived mutant cells…

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Array Tomography for SEM 3D Reconstruction

Array Tomography for SEM 3D Reconstruction Speakers Frank Assen Robert Ranner Tutorial abstract Array tomography (AT) is a 3D image reconstruction technique for high resolution, quantitative analysis of biological structures. For optimal results, ultrathin and ordered sections are an absolute requirement. In this webinar you will get tips and tricks to optimize the workflow of…

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Expanding the Limits of Electron Microscopy Sample Preparation with the Leica EM ICE High Pressure Freezer

Expanding the Limits of Electron Microscopy Sample Preparation with the Leica EM ICE High Pressure Freezer In this tutorial you will learn: Improved vitrification: Specimen vitrification without synchronisation fluid Vitrification strategies: Optimized freezing for different techniques Light and electrical stimulation: Dissect cellular processes with millisecond precision A look into the future: freezing of crystals Speakers…

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Live Cell Isolation by Laser Microdissection

Live Cell Isolation by Laser Microdissection In this tutorial, you will learn: How to best prepare your specimen for live cell isolation by laser microdissection How to optimize your laser microdissection workflow How to avoid common pitfalls of this technique Speakers Dr. Oleg Podgorny Tutorial abstract Laser microdissection is a tool for the isolation of…

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LAS X Navigator Tutorial: Navigating live cell imaging

LAS X Navigator Tutorial: Navigating live cell imaging Speakers Dr. Avi Jacob Tutorial abstract Join Dr. Avi Jacob as he introduces you to the Leica LAS X Navigator, a software module that makes multiparametric acquisition and data review of large data sets easier than you ever thought possible! In this tutorial, Dr. Jacobs will discuss:…

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Super-resolved STED spectroscopy: New insights into molecular membrane dynamics

Super-resolved STED spectroscopy: New insights into molecular membrane dynamics Speakers Christian Eggeling Tutorial abstract Molecular interactions are key in cellular signalling. They are often ruled or rendered by the mobility of the involved molecules. We present different tools that are able to determine such mobility and potentially extract interaction dynamics. Specifically, the direct and non-invasive…

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Precise and lightning-fast spectral Fluorescence Lifetime Imaging at video rate integrated in a high-end confocal microscope

Precise and lightning-fast spectral Fluorescence Lifetime Imaging at video rate integrated in a high-end confocal microscope Speakers Kees JalinkProfessor, The Netherlands Cancer Institute Tutorial abstract Förster (Fluorescence) Resonance Energy Transfer (FRET) has become a powerful tool to study protein-protein interactions and signal transduction in living cells. FRET is commonly read out either by detecting the…

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How to Conduct Localized Proteomics of Microscopic Regions

Precise and lightning-fast spectral Fluorescence Lifetime Imaging at video rate integrated in a high-end confocal microscope In this tutorial, you will learn: How to perform isolated proteomics of microscopic regions of interest How to use this technique with FFPE tissue How proteomics can help you understand molecular mechanisms in disease Speakers Dr. Eleanor Drummond Tutorial abstract…

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Enhance Your Imaging with Super-Resolution: Improve Spatial Resolution and Increase Signal to Noise Ratio in Difficult Samples

In these informative case studies, you will learn how: HyVolution 2 can improve the spatial resolution you get from your sample, whilst remaining gentle to the fluorophores HyVolution 2 overcomes issues experienced with other imaging modalities, even in difficult samples such as plant material, increasing resolution and signal to noise ratio, thereby facilitating downstream image…

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Revealing Cellular Dynamics with Millisecond Precision – The New Tool That Turned Electron Micrographs into Motion Pictures of Neural Communication

Revealing Cellular Dynamics with Millisecond Precision – The New Tool That Turned Electron Micrographs into Motion Pictures of Neural Communication In this tutorial: What if you can dissect the cellular dynamics with millisecond precision? What if you can unravel the morphological transformation of a neuron millisecond by millisecond using electron microscopy? Could this be even…

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Using FIT probes and Super-Resolution Microscopy to Decipher Steps of mRNP Assembly in Developing Oocytes

In this webinar, presented by Dr. Imre Gaspar of the Developmental Biology Unit at EMBL, Heidelberg, you will learn: Importance of mRNA localization and function of mRNPs. Advantages of using fluorogenic FIT probes to visualize mRNPs in vivo and in fixed specimen. How super-resolution microscopy can identify factors required for mRNP biogenesis. Abstract: All mRNA…

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Leica Microsystems Laser Microdissection – Dissection Perfection

Leica Microsystems Laser Microdissection – Dissection Perfection Speakers Dr. Raffaella Maria Balestrini Dr. Falk Schlaudraff Tutorial abstract Leica Microsystems Laser Microdissection systems are the method of choice for laser-based microscopic sample dissection and collection. Laser Microdissection (LMD) is a microscopic technique for isolating homogeneous, specific and pure targets from heterogeneous samples for downstream analysis (DNA,…

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The Best of Both Worlds: Combining Light Sheet and Confocal Microscopy

Abstract: Living cells and organisms often suffer from the high light intensities that are used in conventional imaging. Light sheet microscopy reduces phototoxic effects and bleaching, by only illuminating a specimen in a single plane at a time whilst the signal is detected in a perpendicular direction. In combination with high-speed cameras for image acquisition,…

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Microscope maintenance: changing and aligning your mercury burner

Mercury burners are one of the most common light sources used in fluorescence microscopes, producing a wide spectrum of wavelengths making them a great light source for viewing your samples. But they do have their issues – one of which is their propensity to break or become damaged if not used and cared for correctly, which…

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Fundamentals of Fluorescence Microscopy

What you will learn from this webinar: How a fluorescence microscope works What resolution you can expect to achieve from different microscopes How to choose the right fluorophore Four key elements that require optimization for fluoresence microscopy Summary: Fluorescence microscopy has become one of the most important tools in the biologist’s toolbox.  In this presentation…

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How to Pick the Right Microscopy Technique

In this webinar you will learn: about the variety of imaging techniques that are available how to pick the right technique to answer your scientific question. Summary: There is a plethora of  microscopy techniques available and it can be daunting to figure out which technique is the right one for you. This webinar is designed…

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Basics of Confocal Microscopy

In this webinar you will learn: the basics of confocal laser scanning microscopy why and when you should use confocal microscopy how to get started with confocal microscopy including a demonstration of the key features on a Leica Microsystems confocal microscope  Summary: Are you a confocal novice but interested in using this technique for your own research…

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