Tips and Tricks for Western Blotting and Antibody Selection
You will learn: How to overcome common Western blot issues Which criteria to use for antibody selection Useful information for your grant preliminary data Western blotting is an important and widely used technique in cell and molecular biology. A Western blot is often used in research to separate and identify proteins based on molecular weight…
Isolation and Culture of Primary Cells from Bone Marrow
Bone marrow stromal cells have been one of the more intensively studied adult stem cell types over the past 50 years. Great therapeutic interest in this adult stem cell type has been based on its complex and diverse biological properties including their ability to support a hematopoietic environment, possess anti-inflammatory qualities, and differentiate down multiple…
Multi-color Fluorescence Immunohistochemistry Using Primary Antibodies Raised in the Same Host Species
Abstract: Simultaneous detection of multiple tissue antigens is one of the most frequently used immunohistochemical (IHC) techniques. In order to avoid cross-reactivity of secondary antibodies with unwanted primary antibodies when doing either dual- or triple-labeling immunofluorescence it is necessary to use primary antibodies raised in different host species such as mouse, rabbit and goat. However,…