Get to grips with two indispensable molecular biology techniques in one practical handbook. Learn the basics of qPCR setup, the advantages of qPCR and RT-qPCR, and how the two approaches differ. Inside, you’ll also find a clear guide to choosing between one-step and two-step qRT-PCR. Use it to decide which PCR application best fits your experiment.

Four ready-to-use tools to help you prep, analyze, troubleshoot, and report qPCR data more reliably. Includes an oligo prep helper, ΔΔCt calculator, troubleshooting reference card, and plain-English guide to 11 essential qPCR papers. Use it to catch common setup, calculation, and interpretation errors before they affect your results.