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Using CRISPR/Cas9 to detect sequences on single DNA molecules with high-speed AFM

Using CRISPR/Cas9 to detect sequences on single DNA molecules with high-speed AFM Join Dr. Jason Reed as he describes a novel method by which endonuclease-inhibited Cas9 can be employed as a programmable biomarker in high-speed atomic force microscopy (HS-AFM) imaging.In this tutorial, you will find: How CRISPR/Cas9 can be used to “flag” alterations and mutations…

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A Start to Finish Guide to Target Gene Validation Using Quantitative RT-PCR

A Start to Finish Guide to Target Gene Validation Using Quantitative RT-PCR Speaker Matthew Mule In this tutorial, you will find: While next generation sequencing enables researchers to unveil expression levels of the entire genome, qRT-PCR remains the gold standard for measuring transcript levels of individual genes for functional studies and for the purposes of…

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The Use of qPCR to Validate Epigenetic Enrichment of Pathogen DNA from Complex Samples and Human DNA from Stool

qPCR is one of the most specific and sensitive tools in molecular biology, allowing the quantification of target DNA molecules present at less than 1 in 106. Next Generation Sequencing (NGS) has similar potential. However, the presence of large amounts of non-target DNA in most clinical or environmental samples precludes easy and inexpensive analysis of…

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Common challenges and solutions in microbiome analysis workflows

Common challenges and solutions in microbiome analysis workflows Elucidating meaningful, unbiased microbial community profiles from complex microbiome samples is challenging.In this tutorial, you will find: the sources of bias throughout the microbiome analysis workflow practical solutions for troubleshooting your techniques new technologies to achieve the most representative and unbiased microbiome profiles Speakers Sven Reister, Ph.D.PCR…

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Long Adapter Single Stranded Oligonucleotide (LASSO) Probes for Massively Multiplexed Cloning of Kilobase-Sized Genome Regions

In this webinar, you will learn how to solve a major problem in creating expression libraries from genome sequences for downstream analyses. Specifically, you will learn: The difference and benefits of LASSO cloning over NGS How LASSO cloning allows for the multiplex cloning of large open reading frames (ORFs) of bacterial, human, and human-microbiome genomes…

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qPCR and RNAseq: The Battle of the Strands!

In this webinar, you will learn how qPCR can be used in combination with new technologies, like RNAseq, and how this will help you in your research. The main aspects to be covered in this webinar include: When you should choose RNA-seq over qPCR alone for expression analysis Why and how you should validate RNAseq…

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Multiplex PCR Technology: What Is It All About?

Join Dr. Karen O’Hanlon Cohrt for a practical tour of multiplex PCR technology, where you will learn the following and much more: Principle behind multiplex PCR technology Popular applications of this technology How to set up this reaction Advantages and disadvantages Multiplex PCR Can Benefit Your Research Dr O’Hanlon Cohrt will discuss the history of…

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Decoding Cancer: Practical Advice for Working with Cellular Heterogeneity

In this webinar, you will learn about the process for genotyping cells—particularly tumor cells—and practical advice for analyzing the resulting data. The main points that we will cover are: Practical advice and methods for sample collection and preparation for your genotyping experiments An overview of the available data analysis methods for your genotyping results Examples…

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All about miRNAs: Practical Tips, Advice, and Applications

In this webinar you will learn the tips and tricks necessary for you to successfully work with miRNAs, and how you can use them to further your research. The main points we will cover are: Tips and practical advice to help you work with miRNAs, including isolation and delivery The biology and mechanism of miRNAs and…

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qPCR Tips: Workflow, Applications and Troubleshooting

In this webinar, you’ll get: Practical advice for sample preparation, qPCR setup and result analyses Guidance on choosing the correct fluorescent labeling system for your qPCR Tips to troubleshoot the most commonly encountered issues in qPCR Join Dr. Karen O’Hanlon Cohrt for a practical tour of the process, applications and practice of qPCR, from start…

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Single-cell Genomics: An Overview and Its Application in Investigating Complex Biological Samples

In this webinar you will learn: The science behind single-cell genomics and its applications How single-cell genomics applies to your research through an example study. In this study, single-cell genomics was used to study single cells from microbes in the environment. Practical advice and tips to help you perform single-cell genomics experiments and analysis of the data…

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Next Generation Sequencing Library Preparation: Concepts, Tips and Tricks

In this webinar, you will learn: The core steps in preparing DNA/RNA NGS libraries An overview of the major strategies for generating NGS libraries Tips and tricks to generate high quality NGS libraries for consistent sequencing results The widespread availability NGS has caused a revolution in genetics and molecular biology research. While several NGS platforms…

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Next-Generation Sequencing Overview: Step By Step Guide to NGS Workflow

In this webinar you will learn: Pre-analytical considerations – how different sample types can cause issues in downstream processing. Sample Preparation for the most common applications (Genomes, Exomes, Targeted Panels) – how choosing the right library construction method is critical to success. There is a big world out there – the many other sample preparation…

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Critical Success Factors for Sample Collection, Storage and Retrieval

In this webinar you will learn: How to maintain specimen integrity when storing tissues, blood and cells. How to store and retrieve specimens from a biobank. Special considerations for preparing formalin-fixed paraffin-embedded (FFPE) tissues for storage. Abstract: Biobanks, or biorepositories, store biological samples that are critical for research in the biomedical fields. Samples from biobanks…

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Liquid Biopsy Sample Handling

In this webinar you will learn: How to sensitively, specifically and rapidly analyze circulating free nucleic acids (cfDNA), circulating tumor cells (CTCs) or exosomes from a blood sample using liquid biopsy About miRNA signatures and how they have the potential to serve as biomarkers for disease pathogenesis, prognosis, and response to treatment. How to overcome…

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Techniques for Isolating Circulating Biomarkers

In this webinar you will learn: Which biomarker you want to isolate Which clinical biofluid or specimen is a good biomarker source A basic understanding of small non-coding RNAs, exosome, and circulating tumor cell biology The challenges associated with isolating these biomarkers Various technologies to overcome these challenges Abstract: To gain a better molecular understanding…

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