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THUNDER Imagers: High performance, versatility and ease-of-use for your everyday imaging workflows.

THUNDER Imagers: High performance, versatility and ease-of-use for your everyday imaging workflows. In this webinar, you will learn: How to get sharp, high quality images from thick 3-dimensional microscope samples How to shorten your imaging workflow from image acquisition to data analysis A broad spectrum of imaging workflows for a range of life science applications…

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Basics of Fluorescence Lifetime Imaging and FLIM Applications to FRET Biosensors

Basics of Fluorescence Lifetime Imaging and FLIM Applications to FRET Biosensors In this tutorial you will learn: How researchers (Anzilotti 2019) used FLIM to elucidate the mechanisms responsible for a B cell deficiency A new approach to measure the cellular function of the Zn2+ transporter ZIP7 in primary mouse cells, comparing wild-type and CRISPR-Cas9 derived mutant cells…

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Array Tomography for SEM 3D Reconstruction

Array Tomography for SEM 3D Reconstruction Speakers Frank Assen Robert Ranner Tutorial abstract Array tomography (AT) is a 3D image reconstruction technique for high resolution, quantitative analysis of biological structures. For optimal results, ultrathin and ordered sections are an absolute requirement. In this webinar you will get tips and tricks to optimize the workflow of…

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Expanding the Limits of Electron Microscopy Sample Preparation with the Leica EM ICE High Pressure Freezer

Expanding the Limits of Electron Microscopy Sample Preparation with the Leica EM ICE High Pressure Freezer In this tutorial you will learn: Improved vitrification: Specimen vitrification without synchronisation fluid Vitrification strategies: Optimized freezing for different techniques Light and electrical stimulation: Dissect cellular processes with millisecond precision A look into the future: freezing of crystals Speakers…

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Live Cell Isolation by Laser Microdissection

Live Cell Isolation by Laser Microdissection In this tutorial, you will learn: How to best prepare your specimen for live cell isolation by laser microdissection How to optimize your laser microdissection workflow How to avoid common pitfalls of this technique Speakers Dr. Oleg Podgorny Tutorial abstract Laser microdissection is a tool for the isolation of…

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LAS X Navigator Tutorial: Navigating live cell imaging

LAS X Navigator Tutorial: Navigating live cell imaging Speakers Dr. Avi Jacob Tutorial abstract Join Dr. Avi Jacob as he introduces you to the Leica LAS X Navigator, a software module that makes multiparametric acquisition and data review of large data sets easier than you ever thought possible! In this tutorial, Dr. Jacobs will discuss:…

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Super-resolved STED spectroscopy: New insights into molecular membrane dynamics

Super-resolved STED spectroscopy: New insights into molecular membrane dynamics Speakers Christian Eggeling Tutorial abstract Molecular interactions are key in cellular signalling. They are often ruled or rendered by the mobility of the involved molecules. We present different tools that are able to determine such mobility and potentially extract interaction dynamics. Specifically, the direct and non-invasive…

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Precise and lightning-fast spectral Fluorescence Lifetime Imaging at video rate integrated in a high-end confocal microscope

Precise and lightning-fast spectral Fluorescence Lifetime Imaging at video rate integrated in a high-end confocal microscope Speakers Kees JalinkProfessor, The Netherlands Cancer Institute Tutorial abstract Förster (Fluorescence) Resonance Energy Transfer (FRET) has become a powerful tool to study protein-protein interactions and signal transduction in living cells. FRET is commonly read out either by detecting the…

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How to Conduct Localized Proteomics of Microscopic Regions

Precise and lightning-fast spectral Fluorescence Lifetime Imaging at video rate integrated in a high-end confocal microscope In this tutorial, you will learn: How to perform isolated proteomics of microscopic regions of interest How to use this technique with FFPE tissue How proteomics can help you understand molecular mechanisms in disease Speakers Dr. Eleanor Drummond Tutorial abstract…

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Enhance Your Imaging with Super-Resolution: Improve Spatial Resolution and Increase Signal to Noise Ratio in Difficult Samples

In these informative case studies, you will learn how: HyVolution 2 can improve the spatial resolution you get from your sample, whilst remaining gentle to the fluorophores HyVolution 2 overcomes issues experienced with other imaging modalities, even in difficult samples such as plant material, increasing resolution and signal to noise ratio, thereby facilitating downstream image…

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Revealing Cellular Dynamics with Millisecond Precision – The New Tool That Turned Electron Micrographs into Motion Pictures of Neural Communication

Revealing Cellular Dynamics with Millisecond Precision – The New Tool That Turned Electron Micrographs into Motion Pictures of Neural Communication In this tutorial: What if you can dissect the cellular dynamics with millisecond precision? What if you can unravel the morphological transformation of a neuron millisecond by millisecond using electron microscopy? Could this be even…

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Using FIT probes and Super-Resolution Microscopy to Decipher Steps of mRNP Assembly in Developing Oocytes

In this webinar, presented by Dr. Imre Gaspar of the Developmental Biology Unit at EMBL, Heidelberg, you will learn: Importance of mRNA localization and function of mRNPs. Advantages of using fluorogenic FIT probes to visualize mRNPs in vivo and in fixed specimen. How super-resolution microscopy can identify factors required for mRNP biogenesis. Abstract: All mRNA…

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Leica Microsystems Laser Microdissection – Dissection Perfection

Leica Microsystems Laser Microdissection – Dissection Perfection Speakers Dr. Raffaella Maria Balestrini Dr. Falk Schlaudraff Tutorial abstract Leica Microsystems Laser Microdissection systems are the method of choice for laser-based microscopic sample dissection and collection. Laser Microdissection (LMD) is a microscopic technique for isolating homogeneous, specific and pure targets from heterogeneous samples for downstream analysis (DNA,…

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The Best of Both Worlds: Combining Light Sheet and Confocal Microscopy

Abstract: Living cells and organisms often suffer from the high light intensities that are used in conventional imaging. Light sheet microscopy reduces phototoxic effects and bleaching, by only illuminating a specimen in a single plane at a time whilst the signal is detected in a perpendicular direction. In combination with high-speed cameras for image acquisition,…

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Microscope maintenance: changing and aligning your mercury burner

Mercury burners are one of the most common light sources used in fluorescence microscopes, producing a wide spectrum of wavelengths making them a great light source for viewing your samples. But they do have their issues – one of which is their propensity to break or become damaged if not used and cared for correctly, which…

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Fundamentals of Fluorescence Microscopy

What you will learn from this webinar: How a fluorescence microscope works What resolution you can expect to achieve from different microscopes How to choose the right fluorophore Four key elements that require optimization for fluoresence microscopy Summary: Fluorescence microscopy has become one of the most important tools in the biologist’s toolbox.  In this presentation…

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How to Pick the Right Microscopy Technique

In this webinar you will learn: about the variety of imaging techniques that are available how to pick the right technique to answer your scientific question. Summary: There is a plethora of  microscopy techniques available and it can be daunting to figure out which technique is the right one for you. This webinar is designed…

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Basics of Confocal Microscopy

In this webinar you will learn: the basics of confocal laser scanning microscopy why and when you should use confocal microscopy how to get started with confocal microscopy including a demonstration of the key features on a Leica Microsystems confocal microscope  Summary: Are you a confocal novice but interested in using this technique for your own research…

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