Steffi Khatol

Steffi Khatol
An enthusiastic researcher currently working in a lab at Birla Institute of Technology and Science (BITS), Pilani as a research assistant deciphering the role of CRISPR-Cas system in enteric bacteria. I gained my Master’s degree in Biotechnology from Jamia Millia Islamia, India, and as a first-hand experience working at the Regional Centre for Biotechnology (UNESCO-RCB) on a project to combat antimicrobial resistance. I also like to write, especially on the topics that make science interesting for common non-scientists.

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Articles by Steffi Khatol

Symbolises decision between boiling lysis (time) and alkaline lysis (efficiency)

Alkaline Lysis vs. Boiling Lysis: Selecting the Ideal Plasmid Isolation Method

BySteffi Khatol

Alkaline lysis is ideal when you need high-purity plasmid DNA for applications like cloning. Boiling lysis is a low-cost alternative suitable for routine screening tasks. However, it generally yields lower-purity DNA and is limited to smaller plasmids. This article breaks down the applications of the two approaches.