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Alkaline lysis is ideal when you need high-purity plasmid DNA for applications like cloning. Boiling lysis is a low-cost alternative suitable for routine screening tasks. However, it generally yields lower-purity DNA and is limited to smaller plasmids. This article breaks down the applications of the two approaches.
Autoclaving something that isn’t autoclavable is usually embarrassing, dangerous, or both! Read this simple guide to autoclavable materials in biology labs to demystify what you can and can’t autoclave.
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