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Feeling victimized by your latest Western blot? Whether you're dealing with no bands, faint bands, multiple bands, high background signal, uneven transfer, or *shudders* smearing, this handy troubleshooting card can help you gain clarity.

Imagine having tube after tube of competent cells for your transformations, all (virtually) free of charge. The way to reach this nirvana is to make your own competent cells. Traditionally this can be a tricky feat, but the procedure laid out in this guide makes it simple and routine.