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Western blotting is a cornerstone technique we all use. But it can go wrong and turn out ugly time and time again. Our Western blot protocol cheat sheet is a reliable source for essential buffer and ECL reagent recipes, an adaptable protocol, and top transfer tips to make every blot beautiful.

Feeling victimized by your latest Western blot? Whether you're dealing with no bands, faint bands, multiple bands, high background signal, uneven transfer, or *shudders* smearing, this handy troubleshooting card can help you gain clarity.