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There are some lab math calculations that almost all molecular biologists need to perform, and often on a regular basis. Make sure you never mess up a solution or miscalculate your DNA concentration again, with our handy lab math cheat sheet. Print it out and stick it on your bench.

This laminate-ready bench card gives you a six-factor decision table covering target count, RNA quantity, throughput, inhibitor risk, priming strategy, and enzyme choice. When a run goes wrong, use the troubleshooting flowchart to work through no amplification, inconsistent Ct, multiple melt peaks, or low yield.