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The New ZEISS LSM 9 Family: Adding the Multiplex Factor to Your Confocal Imaging

In this tutorial on confocal imaging, you will learn how you can:

  • Capture weaker signals—and still get sound, reproducible data.
  • Reach faster volumetric imaging without sacrificing resolution
  • Increase data throughput for your imaging needs

Speaker

Joseph Huff

Solution Manager/Application Development Engineer at ZEISS Microscopy

Tutorial abstract

Learn about the new Multiplex mode for parallel pixel acquisition with the ZEISS LSM 9 family and Airyscan 2. You can now acquire up to 8 superresolution image lines with high signal-to-noise rate in a single sweep. Capture dynamic processes, cellular signaling, molecular trafficking, and diffusion events with real-time superresolution and high SNR.

The new Multiplex mode for Airyscan 2 uses smart illumination and detection schemes for parallel pixel acquisition on a confocal microscope. Scientists can now capture weaker signals, keep their context, and get statistically sound data.

Extending Airyscan imaging to larger model systems with lower expression levels, the new Multiplex mode increases acquisition speeds even further. You get superresolution and a 4 times higher SNR compared to traditional confocals. This novel concept allows rapid volumetric imaging with unprecedented resolution beyond what is available in traditional confocal systems today.

Airyscan 2 provides new data handling concepts, providing 6.6 times smaller data sizes and 5 times faster image reconstruction times. Further, optimized real time acquisition strategies employed with the LSM 9 family enable faster scan speeds for Airyscan 2, allowing higher data throughput.

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