qPCR is one of the most specific and sensitive tools in molecular biology, allowing the quantification of target DNA molecules present at less than 1 in 106. Next Generation Sequencing (NGS) has similar potential. However, the presence of large amounts of non-target DNA in most clinical or environmental samples precludes easy and inexpensive analysis of rare events.
In this webinar, you will learn:
1. The epigenetic differences between microbial and human/animal genomes
2. The use of restriction endonucleases to enrich for either pathogen genomes or the human genome from complex populations.
3. How the use of enrichment and concentration can improve qPCR sensitivity
4. The use of qPCR to validate enrichment from complex samples
5. The use of NGS to validate pathogen enrichment from complex samples