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Isolation and Culture of Primary Cells from Bone Marrow

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Tutorial Video Abstract

Bone marrow stromal cells have been one of the more intensively studied adult stem cell types over the past 50 years.

Great therapeutic interest in this adult stem cell type has been based on its complex and diverse biological properties including their ability to support a hematopoietic environment, possess anti-inflammatory qualities, and differentiate down multiple skeletal lineages. However, much confusion and hype has also been associated with this adult stem cell type as evidenced by its expansive nomenclature.

In the past decade, research in different transgenic animal models has begun to provide a much greater understanding of stromal cells, in terms of their origin, identity, and in vivo biological roles. The culturing of stromal cells also has great significance for applications in regenerative medicine and in elucidating mechanisms of development and disease.

In this webinar, I will review work done by us and others in the field that have provided a greater understanding for their origin, identity, and biological complexity. With that in mind, I will discuss elements of the bone marrow stromal cell culture system. Here I will cover different ways to isolate and culture stromal cells, in vitro assays to study their multipotency and quantify their abundance. Along the way, I will touch upon common misconceptions in the field, debate stem/progenitor cell plasticity, predisposition, and differential response.

I will also discuss the benefit of using different murine transgenic animal models to study stromal cells and their differentiated progeny.

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