Reliable quantification of fluorescence images: from raw data to reproducible results

[SVI] Webinar CJS Oct 12 2018 Reliable quantification of

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Tutorial Presented By

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The Huygens software, from Scientific Volume Imaging, pushes the limits of microscopy image resolution and quality. This is accomplished with the most sophisticated deconvolution algorithms, complemented with an extensive toolbox to correct for additional imaging artefacts. Advanced Huygens 3D visualization and analysis can complete your imaging workflow. Our responsive support team is ready to help you with testing Huygens.


Vincent Schoonderwoert, PhD

Tutorial Video Abstract

In this webinar, you will learn:

  • How to overcome issues with stage drift and crosstalk between channels
  • How to obtain reliable images and reproducible quantification results
  • How to proceed with advanced image analysis after restoring images

During fluorescence image acquisition, many experimental uncertainties are introduced that affect the correct object interpretation and analysis. The blurring and the noise implicit in the image formation are two of the largest sources of experimental trouble. Additional aberrations, such as stage drift, and crosstalk and chromatic aberration between channels, can also affect the imaging. Huygens image deconvolution and restoration is a proven method to revert these issues and recover a more realistic representation of the original object. After restoration of the image, you can proceed with the advanced Huygens analysis options for colocalization, object measurements, and tracking.

This webinar will illustrate how you can make optimal use of the complete Huygens workflow to obtain reliable images and reproducible quantification results, focusing on the advanced analysis options offered by the Huygens software.

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