Resource Support Manager
In this webinar, you will see:
- The optimization of the mouse model generation workflow.
- A direct comparison of in vitro transcribed vs. modified synthetic single-guide RNA (sgRNA).
The development of CRISPR/Cas9 tools has revolutionized the genome-editing field, allowing for the generation of genetically modified mice with increased efficiency and ease. We provide a genome engineering service to produce and validate various knock-out (KO) and knock-in models through our evolving processes. This service has led to the development of a high throughput pipeline for the efficient generation of KO mice, allowing the generation of more than 100 KO projects a year as part of the International Mouse Phenotyping Consortium effort.
Additional pipelines include the generation of bespoke genome-edited mouse models, both as an on-demand service and as part of the Genome Editing Mice for Medicine service. One optimization in our pipeline was the transition from generating sgRNAs in-house using in-vitro transcription to buying in synthetic sgRNAs. We assessed the impact this had on our workflow, and we share these findings in our webinar.