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Optimizing CRISPR mouse model pipelines – modified synthetic sgRNAs

On Demand

Speaker

matthew-Mackenzie-85-85

Matthew Mackenzie

Resource Support Manager
MRC Harwell

In this webinar, you will see:

  • The optimization of the mouse model generation workflow.
  • A direct comparison of in vitro transcribed vs. modified synthetic single-guide RNA (sgRNA).

The development of CRISPR/Cas9 tools has revolutionized the genome-editing field, allowing for the generation of genetically modified mice with increased efficiency and ease. We provide a genome engineering service to produce and validate various knock-out (KO) and knock-in models through our evolving processes. This service has led to the development of a high throughput pipeline for the efficient generation of KO mice, allowing the generation of more than 100 KO projects a year as part of the International Mouse Phenotyping Consortium effort.

Additional pipelines include the generation of bespoke genome-edited mouse models, both as an on-demand service and as part of the Genome Editing Mice for Medicine service. One optimization in our pipeline was the transition from generating sgRNAs in-house using in-vitro transcription to buying in synthetic sgRNAs. We assessed the impact this had on our workflow, and we share these findings in our webinar.

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[Sigma Aldrich] Webinar 4-Nov-20 Optimizing CRISPR Mouse

Image of CRISPR/Cas9 with sgRNA
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