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Next-Generation Sequencing Overview: Step By Step Guide to NGS Workflow


Tutorial Video Abstract

In this webinar you will learn:

  • Pre-analytical considerations – how different sample types can cause issues in downstream processing.
  • Sample Preparation for the most common applications (Genomes, Exomes, Targeted Panels) – how choosing the right library construction method is critical to success.
  • There is a big world out there – the many other sample preparation types that might be of interest.


Next generation sequencing (NGS) is a powerful tool for investigating genome-wide phenomena. It has been used to study epigenetic profiles as part of the ENCODE project. It is being used to understand the genetic basis of both common and rare diseases. The Cancer Genome Atlas (TCGA) used NGS to map hundreds of cancer genes. There is no area of modern genetic research that has not been transformed by the advent of NGS. With continued improvements of throughput and yield, the number of human genomes that will be sequenced in the next few years is staggering.

For all the widespread uses of NGS, there are a variety of ways to end up at your destination, along with some unique challenges along the way. To determine the path, it is best to begin with the end in mind.

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