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Next Generation Sequencing Library Preparation: Concepts, Tips and Tricks

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Tutorial Video Abstract

In this webinar, you will learn:

  • The core steps in preparing DNA/RNA NGS libraries
  • An overview of the major strategies for generating NGS libraries
  • Tips and tricks to generate high quality NGS libraries for consistent sequencing results

The widespread availability NGS has caused a revolution in genetics and molecular biology research. While several NGS platforms and applications exist, all platforms and applications require the generation of DNA or RNA libraries.

The main steps for generating these libraries: 1) fragmenting or sizing the DNA or RNA, 2) attaching oligonucleotides adapters to the ends of the fragments, 3) purifying the library, and 4) quantifying the library for sequencing. While these steps sound simple, a variety of factors can affect NGS library quality. This webinar will not only show you the workflow but also help you overcome common errors and problems. In addition, NGS library preparation is not one-size fits all. Different applications can require different library construction strategies. We will show different strategies for NGS library preparation, such as primer designed panels, fusion primers, and whole genome library preparation— among others.

Good NGS results come from good NGS libraries. This webinar will help ensure that you receive consistent, high quality, and reliable NGS data.

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