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Next Generation Sequencing Library Preparation: Concepts, Tips and Tricks

In this webinar, you will learn the core steps in preparing DNA/RNA NGS libraries. Those are: 1) fragmenting or sizing the target sequences to a desired length, 2) attaching oligonucleotides adapters to the ends of target fragments, 3) purifying the final library product and 4) quantifying the library for sequencing. In addition, you will see different strategies for NGS library preparation, such as primer designed panels, fusion primers and whole genome library preparation among others.

High-throughput sequencing, also known as next-generation sequencing (NGS) has caused a revolution in genetics and research. There are several NGS platforms and also distinct NGS applications. However, the main step which is common in all platforms and applications is the generation of DNA/RNA libraries. The preparation of a NGS library involves the construction of the nucleic acid target (DNA or RNA) into a form that is compatible with the sequencing system to be used.

This webinar will give you an overview of the major strategies and then will focus on tips and tricks to ensure you get good quality libraries and consequently, good sequencing results.