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Markerless and Scarless Microbial Genome Engineering

On Demand Webinar

Speaker

erik-web

Erik Eastlund

Principal Scientist, Gene Editing and Novel Modalities, Merck KGaA, Darmstadt, Germany

Bacterial genome engineering is used for many important biotechnological and biomedical purposes spanning renewable energy to global health. Consequently, there is a high demand for improved tools to manipulate and introduce precise changes in microbial genomes.

Escherichia coli (E. coli) is one of the most widely used microorganisms for metabolic engineering and synthetic biology applications. In this webinar, we introduce an E. coli lambda red-mediated CRISPR/Cas vector system that allows for markerless and scarless high-efficiency genome editing.

This CRISPR/Cas system creates various types of precise genetic alterations by facilitating homology-directed repair of otherwise lethal Cas9-induced double-stranded breaks using a provided donor DNA, which can be double- or single-stranded.

In this webinar, we discuss:

  • An E. coli lambda red-mediated CRISPR/Cas vector system that allows for markerless and scarless high-efficiency genome editing.
  • How to achieve knock-in, knockout, and point mutations with high efficiency.
  • An example of how the CRISPR/Cas vector system enhanced the metabolic production of Kdo2-Lipid A in E. coli.

 

This webinar is sponsored by Merck KGaA, Darmstadt, Germany. The life science business of Merck operates as MilliporeSigma in the U.S. and Canada.

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