Viscosity is one of the main factors which influence diffusion in condensed media. In a cell, microscopic viscosity (microviscosity) can play a role in several diffusion-mediated processes, such as drug delivery, signaling, and mass transport. Previously, alterations in microviscosity in cells and organs have been linked to malfunction; however, mapping microviscosity on a single-cell scale remains a challenge.
Join our upcoming webinar to see how microviscosity and crowding have been imaged inside lipid mono- and bi-layers, in cells and in vivo, using fluorescent probes, called molecular rotors (1,2). Molecular rotors respond to the microviscosity of the local environment through the speed of rotation around a sterically-hindered bond that is viscosity-dependent and strongly affects fluorescence lifetime or spectra of rotors, allowing fluorescence imaging. This approach enables the measurement of both the microviscosity and temperature (2,3) as well as the presence of unusual DNA topologies, G-quadruplexes (4,5), and monitors their temporal changes in real time.
In this webinar, you will discover more about:
- Recent developments in molecular rotor imaging, including genetic and passive targeting of rotors (6);
- Applications of the technique for monitoring disease (7,8);
- How Leica Microsystems microscopy solutions are employed for molecular rotor fluorescence imaging of microviscosity, including fluorescence lifetime imaging microscopy (FLIM).
1. M. K. Kuimova Phys Chem Chem Phys. 2012;14, 12671
2. A. Vyšniauskas et al., Int Rev Phys Chem. 2018;37:2, 259
3. A. Vyšniauskas, et al., Chem Sci. 2015;6, 5773
4. A. Shivalingam, et al., Nat Commun. 2015;6, 8178
5. P. A. Summers, B. W. Lewis, et al., Nat Commun. 2021;12, 162
6. J. E. Chambers et al., ACS Nano 2018;12, 4398
7. M. Kubankova et al., ACS Appl Mater Interfaces 2019;11, 36307
8. J. A. Robson et al, Angew Chem Intl Ed. 2020;59, 21431
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