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Long Adapter Single Stranded Oligonucleotide (LASSO) Probes for Massively Multiplexed Cloning of Kilobase-Sized Genome Regions

Tutorial Presented By

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Lorenzo Tosi

Tutorial Video Abstract

In this webinar, you will learn how to solve a major problem in creating expression libraries from genome sequences for downstream analyses. Specifically, you will learn:

  • The difference and benefits of LASSO cloning over NGS
  • How LASSO cloning allows for the multiplex cloning of large open reading frames (ORFs) of bacterial, human, and human-microbiome genomes
  • How this technology can impact our understanding of disease, drug discovery, precision medicine, and so much more

Join Dr. Lorenzo Tosi as he describes LASSO cloning, a technique that will allow for the expression of an entire set of proteins from any organism in more rapid and cost-effective manner.

The development of tools and techniques designed to sequence and understand genomes has advanced our understanding of the world at large. However, our ability to analyze the function of expressed molecules from these genomes is lagging. Even with the advent of next generation sequencing (NGS), which has changed the landscape of genomic sequencing through increased accuracy and speed, our experiments are still limited by the length of DNA targets being read.

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