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Discover a new light sheet microscope for Multiview imaging of living and cleared specimens

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Join Dr Courtney Akitake as she illuminates how Light Sheet Fluorescence Microscopy (LSFM) offers a compelling alternative to traditional epi-illumination techniques, and how this can provide fast and efficient 3D imaging within whole live organisms and large tissue explants.

In this tutorial, you will find:

  • A brief introduction to light sheet microscopy
  • A short overview of optical clearing techniques
  • A discussion on how common challenges of cleared tissue imaging can be solved using LSFM
  • An exploration of several tools for micro- to meso-scale imaging, including a variety of long working distance objectives.

Abstract

Light Sheet Fluorescence Microscopy (LSFM) is a powerful alternative to traditional epi-illumination techniques, especially for 3D imaging within whole live organisms and large tissue explants. By selectively illuminating the observed optical section with a thin sheet of light, photo-damage and bleaching are reduced to a minimum, making light sheet microscopy ideal for nondestructive imaging of fragile samples. Due to the speed and efficiency of image acquisition, LSFM is particularly well suited for volume imaging of samples that have been rendered transparent through various tissue-clearing methods.

This webinar will include a brief introduction to light sheet microscopy and a short overview of optical clearing techniques. We will demonstrate how common challenges of cleared tissue imaging can be solved with features such as flexible sample mounting and the ability to accommodate different optical clearing agents. We will also explore several tools for micro- to meso-scale imaging, including a variety of long working distance objectives, that make light sheet microscopy an extremely versatile technique for imaging cleared tissues.

Sponsored by

Zeiss Microscopy

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Speaker Details

Courtney Akitake, Ph.D

Product Marketing Manager - Lightsheet

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