Current efforts at reprogramming T cells for therapeutic purposes rely on using recombinant viral vectors. Unfortunately, viral vectors do not target transgenes to specific genomic sites. Moreover, the manufacturing and testing of effective viral vectors is often a lengthy and expensive process, which slows research progress and clinical use. However, recent studies have shown that re-engineering T cells in a specific and efficient manner is possible using homology-directed repair (HDR). In this webinar, you will learn:
- The advantages of using HDR versus recombinant viral vectors when modifying T cell genomes
- How long double-stranded and single-stranded DNA can serve as a non-viral HDR template
- A novel method that allows for the insertion of large DNA sequences (>1Kb) without a virus!