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Fundamentals of Fluorescence Microscopy

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Leica Microsystems is a world leader in providing innovative microscopy, camera and software solutions for imaging and analysis of macro-, micro- and nanostructures.


James Jonkman

Tutorial Video Abstract

What you will learn from this webinar:

  • How a fluorescence microscope works
  • What resolution you can expect to achieve from different microscopes
  • How to choose the right fluorophore
  • Four key elements that require optimization for fluoresence microscopy


Fluorescence microscopy has become one of the most important tools in the biologist’s toolbox.  In this presentation I will lay down a foundation for this topic by discussing: How fluorescence microscopes work, choosing fluorophores, and what resolution you can expect to achieve with your microscope.  I will end by describing four key elements that each must be optimized to form a good fluorescence microscope.

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