Understanding molecular interactions in living cells is one of the key elements to deciphering the molecular mechanisms underpinning most cellular functions. The gold standard for studying protein-protein interaction is Förster resonance energy transfer (FRET).
Although there are several approaches to demonstrate FRET in biological samples, using fluorescence lifetime imaging microscopy (FLIM) allows for a straightforward quantification of FRET based on the behavior of donor-only fluorescence.
Join Dr. Padilla-Parra for this webinar to learn about some of the limitations of classical FRET approaches, how much information you can harness from FRET, and how lifetime-based non-fitting approaches such as minimal fraction of interacting donors (mFD) can provide a direct readout of protein-protein interactions in the cellular environment over time.
After the presentation, join Dr. Roberti for a live showcase demonstration of the STELLARIS 5 confocal system with an integrated WLL, combined with the proprietary Acousto-Optical Beam Splitter (AOBS) and new Power HyD S detectors. Together with the new and unique TauSense technology, STELLARIS 5 sets a new standard for the quality of images and quantity of information generated.
In this webinar, you will learn:
- how to investigate protein-protein interaction in live samples;
- ways to get the best out of FRET using fluorescence lifetime information;
- how non-fitting approaches can be powerful tools for functional imaging;
- what the minimal fraction of interacting donors (mFD) can tell you about molecular interactions in cells;
- practical insights into using the STELLARIS 5 confocal system.