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Understanding and Designing Flanking Homology DNA Assembly Experiments

Join us in this webinar on demystifying DNA assembly.

In this tutorial, you will find:

  • How flanking homology DNA assembly methods work
  • How to use web-based software to design experimental methods for flanking homology DNA assembly methods
  • How synthetic DNA fragments fit in to the DNA assembly process



Karan K. Shah

Tutorial abstract

Gibson Assembly steps

Gibson Assembly Scheme

Dr. Nathan Hillson will discuss methods in flanking homology DNA assembly, including Gibson, In-Fusion, and yeast TAR assembly—amongst many other related methods. Current DNA assembly methods offer many advantages over traditional (multiple cloning site, digestion/ligation) approaches, including the ability to assemble multiple fragments at once, the lack of a necessary specific restriction enzyme, and time commitment.

One part of DNA assembly is designing these experimental methods. To that end, Dr. Hillson will provide a demonstration of how to use web-based software to automate and optimize the design of protocols for these methods.

Finally, Dr. Hillson will explain how synthetic DNA fragments fit in to the DNA assembly process and how this relates to your work.

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[Genscript] Webinar CJS Nov 2017 Understanding and Designing

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