Understanding and Designing Flanking Homology DNA Assembly Experiments

[Genscript] Webinar CJS Nov 2017 Understanding and Designing

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GenScript is the leading gene, peptide, protein and antibody research partner for fundamental life science research, translational biomedical research, and early stage pharmaceutical development. Since our establishment in 2002, GenScript has exponentially grown to become a global leading biotech company that provides life sciences services and products to scientists over 100 countries worldwide. During our tenure we have built the best-in-class capacity and capability for biological research services encompassing gene synthesis and molecular biology, peptide synthesis, custom antibodies, protein expression, antibody and protein engineering, and in vitro and in vivo pharmacology – all with the goal to Make Research Easy.

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Nathan Hillson

Tutorial Video Abstract

Join us in this webinar on demystifying DNA assembly. In this webinar, you will learn:

  • How flanking homology DNA assembly methods work
  • How to use web-based software to design experimental methods for flanking homology DNA assembly methods
  • How synthetic DNA fragments fit in to the DNA assembly process
Gibson Assembly steps

Gibson Assembly Scheme


Dr. Nathan Hillson will discuss methods in flanking homology DNA assembly, including Gibson, In-Fusion, and yeast TAR assembly—amongst many other related methods. Current DNA assembly methods offer many advantages over traditional (multiple cloning site, digestion/ligation) approaches, including the ability to assemble multiple fragments at once, the lack of a necessary specific restriction enzyme, and time commitment.

One part of DNA assembly is designing these experimental methods. To that end, Dr. Hillson will provide a demonstration of how to use web-based software to automate and optimize the design of protocols for these methods.

Finally, Dr. Hillson will explain how synthetic DNA fragments fit in to the DNA assembly process and how this relates to your work.

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