Using a single depletion laser avoids laser misalignment and bleaching issues during stimulated emission depletion (STED) microscopy but limits the available fluorophores for use. Imaging with five fluorochromes using one depletion laser is possible using a fluorescence-lifetime imaging microscopy (FLIM)-STED approach to separate dyes with similar emission spectra but different lifetimes.
In this webinar, you with discover:
- The benefits and limitations of using a single depletion laser in STED microscopy
- How using a FLIM-STED approach overcomes issues of misalignment and bleaching
- Five-color FLIM-STED imaging using a single depletion laser
- The possibilities of eight-color FLIM-STED using a single depletion laser