In this webinar you will learn:
- Improved vitrification: Specimen vitrification without synchronisation fluid
- Vitrification strategies: Optimized freezing for different techniques
- Light and electrical stimulation: Dissect cellular processes with millisecond precision
- A look into the future: freezing of crystals
Plunge freezing and cryo imaging of proteins and complexes have revealed new details in understanding the machinery of the cell and how molecules are involved in cellular processes. However, most eukaryotic cells and tissue samples cannot be plunge frozen because of the rapid decay of the cooling rate within the sample during freezing. High pressure freezing, on the other hand, is currently the main approach to vitrify larger samples (up to 200 µm) and to capture the intrinsic changes in fine structure or cellular dynamics. To further improve its cryo solutions, Leica developed a new cryo platform: the EM ICE. This new generation cryo platform combines speed, reliability and flexibility to facilitate research in various scientific fields.
Dr. Julia KönigProduct Manager EM sample preparation for cryo workflows, Leica Microsystems
Julia König was born in Germany and studied Biology at Dresden University of Technology. For her Diploma and PhD thesis she joined the lab of Thomas Mueller-Reichert at the Max Planck Institute of Molecular Cell Biology and Genetics and the Medical Faculty at Dresden University of Technology. There she used correlative light and electron microscopy as well as electron tomography to analyse the ultrastructure of Nematode embryos during different stages of cell division. After finishing her PhD in 2015, she joined the Electron Microscopy Facility of Lucy Collinson at the Francis Crick Institute in London, working on a wide variety of electron microscopy projects as well as methods and workflow development. In January 2018, she joined Leica Microsystems as a product manager for EM sample preparation for cryo workflows.
Dr. Frédéric LerouxAdvanced workflow specialist, Leica Microsystems
Frédéric Leroux completed his Master degree in Biology in 2007 at the University of Ghent where he gained experience in biological EM sample preparation. In 2008, he moved to the physics department at the University of Antwerp where he started his PhD. At the EMAT research group he specialized in advanced electron microscopy of composite materials. 2016, he joined Leica Microsystems as Application Specialist Nanotechnology EMEA.
He received his PhD in 2012. After 2 years as a postdoctoral researcher he became EM sample preparation specialist at EMAT. He thereby uses his multidisciplinary background and broad microscopy experience to improve EM sample preparation of a variety of materials (polymers, composites, biological and industrial materials).