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Mouse Whole-Brain Volume Electron Microscopy for Cellular Connectomics – Enabling Large-Scale SEM Projects with ZEISS MultiSEM

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As the world's only manufacturer of light, X-ray and electron/ion microscopes, ZEISS offers tailor-made microscope systems for 3D imaging in biomedical research, life sciences and healthcare. A well-trained sales force, an extensive support infrastructure and a responsive service team enable customers to use their ZEISS microscopes to their full potential.


Anna Lena Eberle, PhD

Shawn Mikula

Tutorial Video Abstract

Recent advances in high-throughput multi-beam scanning electron microscopy (EM) and mouse whole-brain EM preparation and collection on tape (“Brain-on-Tape”) have resulted in substantial progress towards a nano-scale map of the whole mouse brain. These maps can be used to determine how individual neurons are synaptically connected and can be used to reconstruct the precise “wiring diagram” of the whole mouse brain. We discuss the methods, recent results and remaining challenges. The ZEISS MultiSEM family features 61 or even 91 electron beams scanning in parallel, resulting in unprecedented imaging speed. This finally enables extremely large-scale electron microscopy projects such as the mapping of the brain’s neural networks at high resolution. This talk will outline the operation principle of the technology and give an overview of ongoing further application developments.

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