Quantcast

Live Webinar Date: 06 December, 2017

How to Conduct Localized Proteomics of Microscopic Regions

Start Time:

London Time: 4:00 pm
Boston Time: 11:00 am
San Diego Time: 08:00 am

Webinar Summary

In this webinar, you will learn:

  • How to perform isolated proteomics of microscopic regions of interest
  • How to use this technique with FFPE tissue
  • How proteomics can help you understand molecular mechanisms in disease

We recently developed a technique that allows localized proteomics of microscopic regions of interest, such as specific cell types or neuropathological features of disease. This technique uses laser capture microdissection (LCM) to isolate regions/cells of interest followed by label-free quantitative mass spectrometry (LC-MS). Importantly, we optimized this technique to use formalin-fixed paraffin embedded (FFPE) tissue, so that archived human tissue specimens collected at autopsy could be used. This is a particular advantage of our methodology, as the vast majority of human tissue specimens are FFPE blocks, which are currently an underutilized, but exceptionally valuable resource for medical research. We have successfully used this technique to analyze the proteome of neuropathological features that define Alzheimer’s disease (amyloid plaques and neurofibrillary tangles), as well as specific populations of neurons that are vulnerable in AD. Going forward, the use of localized proteomics has the potential to greatly increase our understanding of the molecular mechanisms that underlie AD. More broadly, this technique could be used to analyze regions or cells of interest isolated from any FFPE tissue, and therefore could be widely used to examine disease pathogenesis across a broad spectrum of diseases.

By registering for this webinar you are opting in to receive communications from the organizers and sponsors.

Sponsored By

Presented By

Dr. Eleanor Drummond

Research Assistant Professor, NYU School of Medicine

Live Webinar Date: 06 December, 2017

How to Conduct Localized Proteomics of Microscopic Regions

Start Time:

London Time: 4:00 pm
Boston Time: 11:00 am
San Diego Time: 08:00 am
Share1
Tweet
Share
+1