X-Ray Microscopy (XRM) is a relatively new technique that combines the geometric magnification of traditional micro-CT with the optical magnification of light microscopy. The Zeiss Versa XRM system allows an unprecedented view inside samples varying in size from the mesoscale (cm) to the microscale (um) at consistently sub-micron image resolutions. This webinar will focus on the biological applications of X-Ray microscopy, covering the imaging of calcified structures (such as bone) to soft tissues such as the intervertebral disc to visualizing blood vessel using vascular tracing agents. The basics of sample preparation will be covered as well as the pros and cons of different imaging conditions. Finally, a sneak view will be provided into using XRM to spatially target, in three-dimensions, tissue specific structures in a whole organism for 3D ultrastructural imaging using Focused Ion Beam – Scanning Electron Microscopy (FIB-SEM) using the ATLAS 5 Correlative Workspace.By registering for this webinar you are opting in to receive communications from the organizers and sponsors.
Dr. James FitzpatrickScientific Director of the Washington University Center for Cellular Imaging (WUCCI) and Associate Professor of Cell Biology & Physiology, Neuroscience, and Biomedical Engineering.
James Fitzpatrick, Ph.D., joined the School of Medicine on June 1st 2015 as the inaugural Scientific Director of the Washington University Center for Cellular Imaging (WUCCI) and as an Associate Professor of Anatomy & Neurobiology and Cell Biology & Physiology. Prior to his appointment, he was Senior Director of Biophotonics and Strategic Technology Initiatives at the Salk Institute for Biological Studies in La Jolla, CA.
Dr. Fitzpatrick completed his undergraduate studies in Chemistry at Kings College, London and undertook graduate training in optical physics at the University of Bristol also in the United Kingdom. During his Ph.D. he designed and developed a novel injection seeded optical parametric oscillator (OPO) laser system for the study of nuclear hyperfine structure in the excited electronic states of gas phase free radical species. After completing his doctorate at the University of Bristol, he moved to the United States as a post-doctoral fellow at the University of Pittsburgh in Pennsylvania. There he shifted his focus to study biological molecules such as peptide mimics and their micro-solvated clusters in the gas-phase using high-resolution fluorescence spectroscopy. In his second post-doc at Carnegie Mellon University, also in Pittsburgh, he spent his time studying protein-protein interactions using tools such as fluorescence microscopy and fluorescence correlation spectroscopy (FCS). After completing his post-doctoral training, he joined the Carnegie Mellon National Technology Center for Networks and Pathways, an NIH funded Roadmap initiative whose mandate was to develop fluorescent probe and imaging informatics technologies to study networks and pathways in living cells.
Dr. Fitzpatrick’s primary research interests lie in the integration and application of multi-scale optical and charged particle imaging technologies -- specifically, biological applications of ion microscopy, development of correlative 3D light and electron microscopy approaches, and new computational tools to visualize and manipulate large-scale multi-dimensional datasets.