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Measuring Extracellular Vesicles by Flow Cytometry: Challenges and Prospects

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Novus Biologicals was founded in the summer of 1996 as a company to license, produce and market antibodies to support niche and emerging areas of research. From Western blots on the kitchen table to a full lab and 100,000 plus products, we have come a long way from our niche antibody strategy.


John Nolan

Tutorial Video Abstract

In this webinar you will learn:

  • Key features of extracellular vesicles (EVs).
  • Fundamental principles regarding their measurement by flow cytometry.
  • Calibrators and standards for EV measurement by flow cytometry.
  • A new high-resolution flow cytometry approach to measuring EVs.


Extracellular vesicles (EVs) are small (~100 nm) membrane-bound vesicles released from the cell surface and intracellular compartments of all cells. EVs are of great interest as clinical biomarkers due to their abundance in blood, CSF, and other body fluids and because they carry protein and nucleic acids from their cell of origin. However, their small size and heterogeneity in biofluids make EVs very challenging to measure. This workshop will highlight the key features of EVs, the fundemental principles underlying their measurement by flow cytometry, and the appropriate calibrators and standards for EV measurements by flow cytometry. Finally, we will introduce a new high resolution flow cytometry approach to EV measurement using a custom high sensitivity flow cytometer and nanoscale calibration and reference particles.


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