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Automating IHC and RNAscope® ISH for academic research: Insights from equine arteritis virus and COVID-19


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Speaker

An image of Dr. Mariano Carossino

Dr. Mariano Carossino
DVM, PhD, Dipl. ACVM

Assistant Professor (appointed)
Department of Pathobiological Sciences & Louisiana Animal Disease Diagnostic Laboratory
Louisiana State University, School of Veterinary Medicine

Dr. Mariano Carossino is an Assistant Professor at the Louisiana State University, School of Veterinary Medicine. His research is focused on understanding virus-host interactions that govern viral pathogenesis and determine disease outcome, and comparative pathology models to study viral diseases. He also has experience in the development and validation of molecular diagnostic assays, including ISH, for detecting pathogens. Recently, his research has focused on SARS-CoV-2.

Speaker Bio

Immunohistochemistry (IHC) is an established technique that is widely used in research settings to assist in protein expression analysis, cellular localization, abundance, and distribution. Additionally, in situ hybridization (ISH) is widely used for visualizing gene expression and assessing changes in expression.

RNAscope® ISH has become a powerful tool across numerous research fields, revolutionizing the way researchers perform RNA ISH, by facilitating reliable RNA transcript detection in diverse tissue types.

In this webinar, you will discover:

  • An introduction to IHC and ISH, current methodologies and the platforms available;
  • The many benefits of IHC/ISH automation and its use in research;
  • More about optimization of antibodies for designing successful IHC experiments;
  • Insights from several case studies including, chronic wasting disease surveillance, equine arteritis virus, and COVID-19.

For research use only. Not for use in diagnostic procedures.

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[LeicaBio] Webinar 26-Aug-21 Automating IHC and RNAscope

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