Applications of Mass Photometry
In this tutorial, you will find:
- How mass photometry is able to measure the mass of single molecules without labelling.
- The process of using mass as a measurable quantity for the identification and quantification of proteins, and protein interactions.
- A comparison of mass photometry with other types of mass measurements in the study of large protein complexes.
- How to use mass photometry to gain detailed mechanistic insight into the actin assembly pathway on a single-molecule level.
- The underlying imaging capabilities of mass photometry, which make it possible to watch the growth of unlabeled actin filaments in real-time.
Speakers
Prof. Justin Benesch
Dr. Nikolas Hundt
Tutorial abstract
Mass photometry allows for the accurate measurement of mass of single molecules in solution, without the need for labeling.
In this webinar, Prof. Justin Benesch (University of Oxford) will first introduce the mass photometry system. He will discuss examples of mass photometry measurements for large protein complexes ranging from homogeneous, mono disperse oligomers to those that extremely polydisperse and heterogeneous, and draw comparisons with mass measurements obtained from native mass spectrometry and other techniques.
Following this, Dr. Nikolas Hundt (Ludwig-Maximilians-Universität (LMU) München) will share details of one specific use of mass photometry: the real-time visualisation of the nucleation process of actin filaments.
These examples demonstrate how mass photometry can serve as a powerful new tool to study biomolecular interaction and how it generates invaluable insight into the mechanistic details of these reactions.
