Andrew Ravanelli, Ph.D.
Senior R&D Scientist, Genome and Epigenome Editing, Merck KGaA, Darmstadt, Germany
Elliott Meer, Ph.D.
Staff Scientist, 10x Genomics
In this webinar, you will learn:
- Tips and best practices for success for CRISPR experimental set-up from delivery to selection
- Key considerations for single-cell CRISPR screening from library preparation to sequencing
- Data analysis guidance for leveraging single cell CRISPR screening data for mechanistic insights into gene function
CRISPR screening is a powerful approach to interrogate mechanisms underlying development, disease, and therapeutic responses. Single cell CRISPR screens extend this approach by directly linking CRISPR perturbations and single cell gene expression readouts, cell by cell. Profile hundreds of different CRISPR perturbations and detect single-guide RNAs (sgRNAs) with directly linked gene expression phenotypes in hundreds to tens of thousands of cells, without prior knowledge of cell types or markers. Leverage Chromium Single Cell Gene Expression with Feature Barcode technology to simultaneously measure CRISPR perturbations and the resulting transcriptional signature. This technology greatly expands the accessibility, scalability, and resolution of high-throughput functional screens, for mechanistic insights into biology.
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