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14 Ways to Measure Immune Cell Activation

[Astarte] Webinar Sept 19 2018 14 Ways to Measure

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At Astarte Biologics, our mission is to help you get on with discovery. Whether you need extra help in the lab with custom research services and assay development, or are searching for hard-to-find immune cells and reagents, we can help you overcome your research roadblocks.

If you're looking for immune cell products, we've got you covered. Our extensive online inventory makes it easy to find the exact cells and donors you need for your experiments. From B Cells to T Cells and PBMC to Stem Cells, we have a variety of products from healthy and disease-state donors, including Antigen-Specific T Cell you can't find anywhere else. Find it all at Astarte Biologics.

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Speakers

Anne Lodge

Tutorial Video Abstract

Activation of immune cells is the all-important first step in mounting an immune response. Immune cell activation is a popular area of research because so much happens that is key to the downstream goal of fighting infection, cancer, and disease.

There are many ways to measure immune cell activation, and they all have utility. Methods can be grouped into four main categories: Proliferation Assays, Cytokine Measurement, Surface Antigen Expression, and Cytotoxicity.

In this webinar, we’ll discuss specific assays in each of these categories, the joys and pitfalls of each assay, and recommendations on how to choose the best method. You will learn tips and strategies for successful assay development using the following methods:

Proliferation:

  • 3H-Thymidine Uptake
  • Bromodeoxyuridine Uptake (BrdU)
  • ATP Luminescence
  • Fluorescent Dye Reduction (CFSE)

Cytokine Measurement:

  • Multiplex vs. Single Cytokine
  • Choice of Cytokine (IFNg, TNFa, IL-6, IL-1?, etc.)
  • Kinetics of Cytokine Release

Surface Antigen Expression:

  • CD69, CD25, PD-1, etc.
  • Combine with CFSE, Ki67 or BrdU
  • Kinetics are Important

Cytotoxicity:

  • Two-Label Flow Cytometry
  • Calcein AM Dye Release
  • Luciferase Transduced Targets
  • Annexin V