Quantcast
Skip to content

14 Ways to Measure Immune Cell Activation

In this tutorial:

We’ll discuss specific assays in each of these categories, the joys and pitfalls of each assay, and recommendations on how to choose the best method. You will learn tips and strategies for successful assay development using the following methods:

Proliferation:

  • 3H-Thymidine Uptake
  • Bromodeoxyuridine Uptake (BrdU)
  • ATP Luminescence
  • Fluorescent Dye Reduction (CFSE)

Cytokine Measurement:

  • Multiplex vs. Single Cytokine
  • Choice of Cytokine (IFNg, TNFa, IL-6, IL-1?, etc.)
  • Kinetics of Cytokine Release

Surface Antigen Expression:

  • CD69, CD25, PD-1, etc.
  • Combine with CFSE, Ki67 or BrdU
  • Kinetics are Important

Cytotoxicity:

    • Two-Label Flow Cytometry
    • Calcein AM Dye Release
    • Luciferase Transduced Targets
    • Annexin V

Speakers

Anne Lodge

Anne Lodge

CEO of Astarte Biologics

Tutorial abstract

Activation of immune cells is the all-important first step in mounting an immune response. Immune cell activation is a popular area of research because so much happens that is key to the downstream goal of fighting infection, cancer, and disease.

There are many ways to measure immune cell activation, and they all have utility. Methods can be grouped into four main categories: Proliferation Assays, Cytokine Measurement, Surface Antigen Expression, and Cytotoxicity.

Tutorial brought to you by

Astarte Biologics Logo

[Astarte] Webinar Sept 19 2018 14 Ways to Measure

  • Subscribe to Watch this Premium Tutorial

    Get more information from Astarte Biologics and get instant access.
  • ​​All emails include an unsubscribe link. You may opt-out at any time. See our Privacy Policy & Terms & Conditions
  • This field is for validation purposes and should be left unchanged.
Scroll To Top
Share via
Copy link